The Hofmann reagent is a primary test for DMT and LSD. Like Ehrlich, Hofmann reacts with a class of substances called indoles. Hofmann reactions tend to be slower, and with more distinct colours.

Hofmann Reagent Test is one of main reagents for identifying LSD or DMT, but it is also suitable for all lysergamides, tryptamines and more. It is used to rule out 2C-x, DOx, NBOMe or different, less predictable, less desirable, highly toxic dangerous drugs. Multiple reagents are required to detect adulterants and increase analysis precision.